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M94A3250.TXT
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1994-10-25
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Document 3250
DOCN M94A3250
TI Highly LFA-1-expressing U937 subclones showing lower susceptibility to
HIV-1 can lead to continuously stable virus production after long
persistent period.
DT 9412
AU Kameoka M; Okada Y; Fujinaga K; Kimura T; Fujii N; Ikuta K; Inst.
Immunol. Sci., Hokkaido Univ., Sapporo, Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):119 (abstract no. PA0095). Unique
Identifier : AIDSLINE ICA10/94369325
AB OBJECTIVE: Heterogeneous HIV-1 life cycles in monocyte-macrophage
lineage were examined using U937 subclones for understanding the
mechanisms of HIV-1 latent or persistent infection. METHODS: A total of
46 clones (#1-#46) were isolated from U937. The sensitivity to HIV-1
infection was measured by the appearance of HIV-1 phenotypes.
Differentiation levels of the clones were examined before and after
infection. RESULTS: The U937 subclones were classified into three (high,
middle, and low) types by sensitivity to HIV-1 infection. The high
sensitivity correlated with the amounts of extra-chromosomal viral
genome and induction of protein kinase C, but not with the amounts of
adsorbed and integrated viral genomes. The low-type, but not high-type,
clones highly expressing LFA-1 antigens were easily differentiated by
PMA. On the other hand, after infection, the high-type clones were
easily differentiated, whereas the low-type clones did not, and finally
became stable, high virus producers after long latent period. DISCUSSION
AND CONCLUSIONS: Highly LFA-1-expressing clones were differentiated by
PMA, but failed to be differentiated by HIV-1 infection. The sensitivity
of these clones to the infection was low, but finally led to stable,
continuous production of HIV-1. By contrast, the high-type clones
expressing dim LFA-1 were not differentiated by PMA, but easily
differentiated by the infection. This difference could be mediated by
intracellular signaling.
DE Cell Differentiation/DRUG EFFECTS/*PHYSIOLOGY Clone Cells Comparative
Study Human HIV-1/GENETICS/*PHYSIOLOGY Lymphocyte Function-Associated
Antigen-1/*BIOSYNTHESIS Macrophages Monocytes Signal Transduction
Tetradecanoylphorbol Acetate/PHARMACOLOGY Tumor Cells, Cultured Virus
Integration *Virus Replication MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).